dy285b (R&D Systems)

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R&D Systems dy285b
Dy285b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn%CE%B3/Human+IFN-gamma+DuoSet+ELISA/pm42817441-85-9-11
Average 96 stars, based on 394 article reviews

dy285b - by Bioz Stars, 2026-10

96/100 stars

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Concentration Assay:

Article Title: Pioneer factor IRF1 unlocks latent enhancers to rewire chromatin and immunometabolism in inflammatory macrophages
Article Snippet: .. The following day, cells were stimulated with 400 U/mL IFNγ (R&D Systems, Cat. #485-MI-100) for either 0, 1, 3, 6, 12, or 48 h. Thirty minutes before harvest, 4-thiouridine (4SUTP) (Jena Bioscience, Cat. #NU-1156L) was added to each well at a final concentration of 200 μM for metabolic labeling, as previously described ( ). .. The following day, cells were stimulated with 400 U/mL IFNγ (R&D Systems, Cat. #485-MI-100) for either 0, 1, 3, 6, 12, or 48 h. Thirty minutes before harvest, 4-thiouridine (4SUTP) (Jena Bioscience, Cat. #NU-1156L) was added to each well at a final concentration of 200 μM for metabolic labeling, as previously described ( ).

Labeling:

Transduction:

Article Title: From bats to humans: uncovering ISG15 as a new resistance factor in type 1 diabetes
Article Snippet: Human islet microtissues (hIsMTs) (InSphero, MT-04-002-01-60) were generated after dispersion and reaggregation of primary human islets from a single donor (UNOS ID #ALIR128; male; 41y.o. .. ; Hispanic; BMI 27.77; positive serologies: CMV IgG, EBV IgG; HbA1c: 5.3%; cause of death: head trauma). hIsMTs were transduced with AAVs encoding specific shRNA targeting ISG15 (siISG15 #1 sequence) from Vector Biolabs during aggregation for 5 days in Akura hanging drop plates (InSphero, CS-PF24) and then released into Akura 96-well plates (InSphero, CS-09-001-00). hIsMTs were cultured in standard culture medium (InSphero, CS-07-005-01) or standard culture medium containing cytokines: TNFα (25 ng/mL, R&D Systems, 10291-TA-050); IL1β (5 ng/mL, Sigma, H6291-10UG); IFNγ (25 ng/mL, R&D Systems, 10067-IF-100) and IFNα (10 ng/mL, Peprotech, 300-02AA) starting at day 9 after reaggregation, for 6 days. ..

shRNA:

Sequencing:

Cell Culture:

Article Title: Development and validation of a potency assay matrix for optimized and consistent manufacture of clinical mesenchymal stem/stromal cells
Article Snippet: .. Monocytes were cultured by seeding 80x10 3 cells in RPMI/10%FBS in 96-well plates and differentiated to either M1 macrophages using 50ng/mL GM-CSF (Thermo Fisher, 300-03-50UG) and 100ng/mL IFNγ (R&D Systems, 285-IF-100/CF), or M2 macrophages using 50ng/mL M-CSF (ThermoFisher, PHC9504) and 10ng/mL IL-10 (ThermoFisher, 200-10-50UG) or cultured with 40x10 3 MSCs or 50% MSC-CM for 3 days using 96-well plates. .. Where indicated, 2μg/mL M-CSF neutralizing antibody (R&D systems, MAB216-100), 2μg/mL MerTK antibody (R&D Systems, AF891), 50nM CSF1R inhibitor (Selleckchem, S7725) or 2.5μM MerTKi (Selleckchem, S7342) was added.

Enzyme-linked Immunosorbent Assay:

Article Title: Sympathetic neurons control adaptive immunity in response to Streptococcus pneumoniae infection by regulating T cell and B cell effector function
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used according to the manufacturer’s instructions, and the plates were analyzed with a BioTek Synergy H1 or BioLegend MiniELISA plate reader. .. The following commercially available ELISA kits were used: Norepinephrine (Novus Biologicals, #197247), Acetylcholine (Thermo Fisher Scientific, #EEL012), TNFα (R&D Systems, #DY410-05), IFNγ (R&D Systems, #DY485-05) FasL (R&D Systems #MFL00). .. For specific IgG determination, crude S. pneumoniae (12.53 μg/ml ATCC #49619) extract was incubated in 96-well plates overnight at 4°C, then blocked with 2%BSA for 2h at RT.

Article Title: Mincle-dependent Th17 adjuvanticity requires TNFR1 signaling in myeloid cells
Article Snippet: .. For this purpose, the following ELISA kits were used according to the manufacturer’s instructions: IFNγ (R&D Systems, DY485), IL-17 (R&D Systems, DY421), IL-10 (R&D Systems, DY417), and IL-6 (R&D Systems, DY406). .. After blocking with anti-mouse CD16/32 antibody (eBioscience) the dead cells were stained with the Fixable Viability Dye eFluor 506 (Invitrogen 65-0866-14).

Recombinant:

Article Title: MAPK Pathway Mutations Emerge in Mutant IDH1 Inhibitor–Resistant Cholangiocarcinoma and Attenuate the IFN Response
Article Snippet: Human mIDH1 ICC cell line SNU1079 was treated for 5 days with either dimethyl sulfoxide (DMSO) or 1 mmol/L ivosidenib (MedChemExpress, cat. #HY-1876). .. IFNγ (10 ng/mL; Recombinant Human IFN-gamma Protein, R&D Systems, cat. #285-IF-100) was added during the final 2 days of treatment. ..

Article Title: MAPK Pathway Mutations Emerge in Mutant IDH1 Inhibitor–Resistant Cholangiocarcinoma and Attenuate the IFN Response
Article Snippet: .. On the final day, cells were also treated with 100 nmol/L MRTX1133 or RMC-7977 and then 10 ng/mL IFNγ (Recombinant Human IFN-gamma Protein, R&D Systems, cat. #285-IF-100; or Recombinant Mouse IFN-gamma Protein, R&D Systems, cat. #485-MI-100). ..

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